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mCherry antibody

Antigen

mCherry

Clonality Polyclonal
Host

Rabbit

Application
Western Blotting (WB)
1 reference available
Catalog no. ABIN411533
Quantity 100 µg  (0.5 mg/ml)
Price 245.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Characteristics 100 ug affinity purified rabbit anti-mCherry polyclonal antibody. Tested in Western blot analysis. Other applications have not been determined. Recombinant mCherry Red Fluorescent Protein (Cat.# 4993-100) can be used as a positive control.
Gene ID 262089840
Immunogen E. coli expressed recombinant mCherry
Isotype IgG  (Matching secondary antibodies)
Description MCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence intensity. mCherry protein is a 28.8 kDa monomer with 256 amino acid.

Application Details

Application Notes Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant mCherry (Cat.#: 4993-100) can be used as a positive control.
Concentration 0.5 mg/ml
Purification Affinity Purified Antibody
Buffer 100 µg (0.5 mg/ml) affinity purified rabbit anti-mCherry polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Storage Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
Restrictions For Research Use only

Publications

Publications Yu, Götz: "Cell Wall Antibiotics Provoke Accumulation of Anchored mCherry in the Cross Wall of Staphylococcus aureus." in: PLoS ONE, Vol. 7, Issue 1, pp. e30076, 2012 (PubMed).