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Antibodies
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anti-Nanog Homeobox Pseudogene 8 (NANOGP8) antibody
Nanog Homeobox Pseudogene 8 (NANOGP8) antibody
| Antigen |
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| Synonyms |
ENK, ecat4, 2410002E02Rik, PN8, NANOG, NANOGP1, MGC119250, nanog |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (109), Mouse (Murine) (31), Rat (Rattus) (24), Dog (Canine) (5), Chicken (3), Cow (Bovine) (3), Rabbit (2), Zebrafish (Brachydanio rerio) (2), Goat (1), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Application
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Western Blotting (WB) (79), Immunofluorescence (IF) (51), Flow Cytometry (FACS) (37), ELISA (36), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
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| Catalog no. |
ABIN411539 |
| Quantity |
100 µg (0.25 mg/ml) |
| Price |
285.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human. Tested in ELISA and WB.
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Alternative name
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Nanog
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Gene ID
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79923 and 100293888
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Immunogen
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His fusion recombinant protein of human FOXP3
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Isotype
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IgG (Matching secondary antibodies)
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Description
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NANOG is a transcription regulator involved with inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extra-embryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.
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Specificity
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The antibody detects a ~ 34 kDa band, corresponding to the expected molecular mass of Nanog on immunoblots.
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Application Notes
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The antibody can be used for ELISA (0.25 µg/ml) and Western Blotting (2.5 – 5.0 µg/ml).
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Concentration
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0.25 mg/ml
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Purification
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Prepared by Saturated Ammonium Sulfate (SAS) precipitation
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Buffer
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100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Embryogenesis, Embryonic stem cells, Cell Cycle
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Restrictions
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For Research Use only
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Alternatives for antigen "Nanog Homeobox Pseudogene 8 (NANOGP8)", type "Antibodies"
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Hosts
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Rabbit (83), Mouse (24), Goat (8)
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Reactivities
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Human (109), Mouse (Murine) (31), Rat (Rattus) (24), Dog (Canine) (5), Chicken (3), Cow (Bovine) (3), Rabbit (2), Zebrafish (Brachydanio rerio) (2), Goat (1), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (79), Immunofluorescence (IF) (51), Flow Cytometry (FACS) (37), ELISA (36), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
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Conjugates
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Biotin (7), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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C-Term (3), Center (3), N-Term (2), S285 (2), S71 (1)
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Alternatives 
