Nestin (NES) antibody
| Antigen |
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| Synonyms |
FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (62), Mouse (Murine) (30), Rat (Rattus) (29), Cow (Bovine) (5), Rabbit (5), Horse (Equine) (4), Pig (Porcine) (4), Dog (Canine) (3), Chicken (1), Chimpanzee (1), Hamster (1), Monkey (1), Sheep (Ovine) (1)
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Immunofluorescence (IF) (47), Flow Cytometry (FACS) (44), Western Blotting (WB) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (10), Immunoprecipitation (IP) (9), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (IHC) (7), Immunocytochemistry (ICC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN411540 |
| Quantity |
100 µg (0.25 mg/ml) |
| Price |
285.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human and mouse. Tested in ELISA, WB, IHC and IFC.
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Alternative name
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Nestin
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Gene ID
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10763
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Immunogen
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Synthetic peptide selected from the N-terminal region of human Nestin
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, Nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
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Specificity
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The antibody detects a ~ 177 kDa band, corresponding to the expected molecular mass of Nestin on immunoblots.
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Application Notes
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The antibody can be used for ELISA (0.25 µg/ml), Western Blotting (2.5 – 5.0 µg/ml), Immunohistochemistry (2.5 – 5.0 µg/ml) and Immunofluorescence (5 - 25 µg/ml).
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Concentration
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0.25 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Angiogenesis, Lineage Markers
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Restrictions
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For Research Use only
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Alternatives for antigen "Nestin (NES)", type "Antibodies"
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Hosts
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Rabbit (69), Mouse (16), Chicken (1), Goat (1)
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Reactivities
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Human (62), Mouse (Murine) (30), Rat (Rattus) (29), Cow (Bovine) (5), Rabbit (5), Horse (Equine) (4), Pig (Porcine) (4), Dog (Canine) (3), Chicken (1), Chimpanzee (1), Hamster (1), Monkey (1), Sheep (Ovine) (1)
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Applications
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Immunofluorescence (IF) (47), Flow Cytometry (FACS) (44), Western Blotting (WB) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (10), Immunoprecipitation (IP) (9), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (IHC) (7), Immunocytochemistry (ICC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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C-Term (4), AA 1484-1500 (1), AA 544-776 (1)
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