NIK antibody

Antigen: NIK

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Hamster

Application: Western Blotting (WB)

Catalog no.: ABIN411546

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-NIK polyclonal antibody. Reacts with human, mouse , rat, hamster and dog. Detects ~105 kDa endougeneous NIK in Western blot analysis. Other applications have not been determined. Blocking peptide (Cat.# 3193RBP-50) is available separately.

Gene ID: 9020

Immunogen: Synthetic peptide surrounding amino acid 927of human NIK

Isotype: IgG  (Matching secondary antibodies)

Description: NIK (NF-kB-inducing kinase) is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK was initially isolated from a human B cell cDNA library and contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-NIK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Transcription Factors

Restrictions: For Research Use only