P21-Activated Kinase 6 (PAK6) antibody

Antigen: P21-Activated Kinase 6 (PAK6)

Synonyms: PAK5, 4732456M09, pak6, PAK6, DKFZp459O104

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Horse (Equine)

Application: Western Blotting (WB)

Catalog no.: ABIN411551

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-PAK6 polyclonal antibody. Reacts with human/mouse/rat/pig/horse. Tested in Western blot analysis. Other applications have not been determined. Blocking peptide (Cat.# 3927BP-50) is available separately.

Alternative name: PAK6

Gene ID: 214230

Immunogen: Synthetic peptide surrounding amino acid 552 of mouse Pak6

Isotype: IgG  (Matching secondary antibodies)

Description: The PAK (p21-activated kinase) family of serine/threonine kinases plays an important role in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, etc. Several mechanisms that induce PAK activation have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. More recently identified members PAK4, PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the regulatory N-terminal region.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~75 kDa of Pak6 in Western blot analysis. Reactivity to other species has not been tested.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Signaling, Kinases/Phosphatases

Restrictions: For Research Use only