Paired Box 6 (PAX6) antibody

Antigen: Paired Box 6 (PAX6)

Synonyms: AN, AN2, MGDA, WAGR, D11S812E, MGC17209, Dey, Sey, AEY11, Pax-6, Gsfaey11, 1500038E17Rik, pax-6, an2, mgda, wagr, pax6a, xpax6, XLPAX6, MGC52531, PAX6, pax6, pax6b, PAX-6

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411553

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-PAX6 polyclonal antibody. Reacts with human/mouse/rat. Other species have not been determined. Tested in Western blot analysis. Blocking peptide (Cat.# 3636RBP-50) is available separately.

Alternative name: PAX6

Gene ID: 25509

Immunogen: Synthetic peptide surrounding amino acid 303 of rat PAX6

Isotype: IgG  (Matching secondary antibodies)

Description: PAX genes encode nuclear transcription factors that may function as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters’ anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Specificity: The antibody recognizes ~32 kDa and 47 kDa isoforms of PAX6 in samples from human, mouse and rat origins. Reactivity to other species has not been tested.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~32 kDa and 47 kDa isoforms of PAX6 in samples from human, mouse and rat origins. Reactivity to other species has not been tested.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Organogenesis, Lineage Markers, Transcription Factors, Neural Stem Cell marker

Restrictions: For Research Use only