Perforin 1 (Pore Forming Protein) (PRF1) antibody

Antigen: Perforin 1 (Pore Forming Protein) (PRF1)

Synonyms: PRF1, P1, PFP, FLH2, PFN1, HPLH2, MGC65093, Pfn, Pfp, Prf-1, Cyta, RATCYTA, MGC108712, LOC443187, perforin, prf1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN411557

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-perforin polyclonal antibody. Reacts with human, mouse, and rat. Tested in Western blto analysis. Per researcher's feedback, the antibody also works well in immunoprecipitation, immunofluorescence and immunohistochemistry. Other applications have not been determined.

Alternative name: Perforin

Gene ID: 50669

Immunogen: Synthetic peptide surrounding amino acid 568 of rat Perforin

Isotype: IgG  (Matching secondary antibodies)

Description: Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~70 kDa Perforin in samples from human, mouse and rat origins. Reactivity to other species has not been determined.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Perforin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Immunology, Innate Immunity, Adaptive Immunity, Inflammation

Restrictions: For Research Use only