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Antibodies
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anti-Pim-1 Oncogene (PIM1) antibody
Pim-1 Oncogene (PIM1) antibody
| Antigen |
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| Synonyms |
PIM, Pim-1, pim2, cb1068, fj47b05, wu:fj47b05, pim1-a, PIM1, pim3, MGC89574, pim-1, PAP-1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunoprecipitation (IP)
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| Catalog no. |
ABIN411562 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
225.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 ug affinity purified rabbit anti-PIM1 polyclonal antibody. Reacts with human, mouse and rat. Detects the 33 kDa PIM1 isoform in Western Blot analysis. Jurkat cell lysate can be used as a positive control. Blocking peptide (Cat.# 3787RBP-50) is available separately.
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Alternative name
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PIM1
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Gene ID
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24649
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Immunogen
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Synthetic peptide surrounding amino acid 296 of rat PIM1
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Isotype
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IgG (Matching secondary antibodies)
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Description
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The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis.
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Application Notes
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Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~33 kDa of PIM1 isoform from samples of human, mouse and rat origins. Reactivity to other species has not been determined.
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.5 mg/ml) affinity purified rabbit anti-PIM1 polyclonal antibody in phosphate (PBS, pH 7.2) containing 30% glycerol, 0.5 % BSA and 0.01% thimerosal.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Cancer, Hematopoietic Progenitors
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Restrictions
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For Research Use only
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Alternatives for antigen "Pim-1 Oncogene (PIM1)", type "Antibodies"
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Hosts
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Mouse (25), Rabbit (15)
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Reactivities
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Human (37), Mouse (Murine) (1), Rat (Rattus) (1)
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Applications
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Enzyme Immunoassay (EIA) (25), Western Blotting (WB) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), ELISA (8), Immunofluorescence (IF) (6), Immunohistochemistry (IHC) (4), Immunocytochemistry (ICC) (1)
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Epitopes
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C-Term (2), internal (1)
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