Protein Tyrosine Phosphatase 1B (PTPN1) antibody

Antigen: Protein Tyrosine Phosphatase 1B (PTPN1)

Synonyms: PTP1B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN411573

Additional Information

Characteristics: 100 µg (0.25 mg/ml) affinity purified antibody. Reacts with human/mouse. Tested in ELISA, Western Blot.

Alternative name: PTP1B

Gene ID: 5770

Immunogen: Recombinant protein encoding amino acids 1-321 of human PTP1B

Isotype: IgG  (Matching secondary antibodies)

Description: PTP1B is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.

Specificity: The antibody detects a ~ 50 kDa band, corresponding to the expected molecular mass of PTP1B on immunoblots.

Application Details

Application Notes: The antibody can be used for ELISA (0.25 µg/ml), Western blotting (0.5 – 2.5 µg/ml).

Concentration: 0.25 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Cardiovascular, Atherosclerosis

Restrictions: For Research Use only