PUMA Bbc3 (0) antibody

Antigen: PUMA Bbc3 (0)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411574

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-PUMA/bbc3 polyclonal antibody. Reacts with human, mouse and rat. Detects PUMA alpha (193 a.a., ~25 kDa) and beta (131 a.a., ~16 kDa) forms in Western blotting. Other applications have not been determined. Blocking peptide (Cat.# 3393RBP-50) is available separately.

Alternative name: PUMA/bbc3

Gene ID: 27113

Immunogen: Synthetic peptide surrounding amino acid 177 of human PUMA

Isotype: IgG  (Matching secondary antibodies)

Description: PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Specificity: The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only