Sp3 antibody

Antigen: Sp3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411588

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-SP3 Transcription Factor polyclonal antibody. Reacts with human/mouse/rat. Detects ~90 Kda band in Western blot analysis. Other applications have not been determined. Blocking peptide (Cat.# 3887BP-50) is also available.

Gene ID: 6670

Immunogen: Synthetic peptide surrounding amino acid 766 of mouse Sp3

Isotype: IgG  (Matching secondary antibodies)

Description: Sp transcription factor family has a highly conserved DNA-binding domain composed of zinc fingers. The Sp family is comprised of Sp1, Sp2, Sp3 and Sp4 that regulates transcription by binding to consensus GC- and GT-box regulatory elements in the target genes. Sp1, Sp3 and Sp4 have a high binding capacity for GC box while Sp2 binds weakly to the GT box. Sp3 has been shown to have to a bifunctional transcription factor that can either stimulates or represses the transcription of various genes.

Specificity: The antibody detects ~86-100 kDa Sp3 in samples from human, mouse and rat origins. Blocking peptide (Cat.# 3887BP-50) is available separately.

Application Details

Application Notes: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. The antibody detects ~86-100 kDa Sp3 in samples from human, mouse and rat origins.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-Sp3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only