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Antibodies
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anti-Sphingosine Kinase 2 (SPHK2) antibody
Sphingosine Kinase 2 (SPHK2) antibody
| Antigen |
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| Synonyms |
C76851, MGC102297, CG2159, CG32484, DmelCG32484, SK2, Sphk2, DKFZp469F224 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (56), Mouse (Murine) (32), Rat (Rattus) (32), Cow (Bovine) (5), Dog (Canine) (3), Horse (Equine) (3), Rabbit (3), Chicken (2), Guinea Pig (1), Monkey (1), Pig (Porcine) (1)
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Application
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Flow Cytometry (FACS) (34), Immunofluorescence (IF) (33), Western Blotting (WB) (21), ELISA (10), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (IHC) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN411590 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
225.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 ug affinity purified rabbit anti-Sphingosine Kinase 2 (SK2, SPK2) polyclonal antibody. Detects 69 kDa Sphingosine Kinase 2. Reacts with human/mouse/rat. Tested in Western Blot. Other applications have not been determined.
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Alternative name
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Sphingosine Kinase 2 (SPK2)
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Gene ID
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308589
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Immunogen
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Synthetic peptide corresponding to residues surrounding amino acid 603 of rat Sphingosine Kinase 2
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Sphingosine Kinase (SphK) is a conserved lipid kinase that catalyzes the phosphorylation of sphingolipid sphingosine to sphingosine-1-phosphate (SIP). SIP receptors coupled to cell surface G protein to regulate cell growth, survival, motility and inflammatory responses. There are two types of SphK, SphK1 and SphK2. SphK1 is found in the cytosol and migrates to the membrane when activated. SphK1 has been associated with cell growth, prevention of apoptosis and cellular transformation. SphK2 is found mainly in the nucleus and in contrast to SphK1, it enhances the rate of apoptosis.
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Specificity
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The antibody recognizes ~69 kDa bands from samples of human, mouse and rat origins. Reactivity to other species has not been tested.
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Application Notes
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Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~69 kDa bands from samples of human, mouse and rat origins. Reactivity to other species has not been tested.
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Restrictions
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For Research Use only
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Alternatives for antigen "Sphingosine Kinase 2 (SPHK2)", type "Antibodies"
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Hosts
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Rabbit (55), Mouse (1)
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Reactivities
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Human (56), Mouse (Murine) (32), Rat (Rattus) (32), Cow (Bovine) (5), Dog (Canine) (3), Horse (Equine) (3), Rabbit (3), Chicken (2), Guinea Pig (1), Monkey (1), Pig (Porcine) (1)
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Applications
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Flow Cytometry (FACS) (34), Immunofluorescence (IF) (33), Western Blotting (WB) (21), ELISA (10), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (IHC) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Flour 488 (1)
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Epitopes
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Long Form (3), N-Term (3), C-Term (2), pThr614 (1)
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Alternatives 
