SSEA-4 antibody
| Antigen |
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| Clonality |
Monoclonal (MC-813-70) |
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Host
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Reactivity
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Application
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Alternatives Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN411593 |
| Quantity |
25 ug (0.5 mg/ml) |
| Price |
110.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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25 ug. This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ (EG) cells) and human embryonic stem (ES) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression. Applications: Flow (Cell Surface): 10 µl/106 cells; IF, ICC, IP and Western blot.
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Gene ID
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N/A
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Immunogen
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Human embryonal carcinoma cell line 2102Ep
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Isotype
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IgG3, kappa chain (Matching secondary antibodies)
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Clone
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MC-813-70
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Description
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This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ (EG) cells) and human embryonic stem (ES) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
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Application Notes
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Flow (cell Surface), IHC, IP, Western Blot
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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25 µg in 50 ul PBS containing 0.05% BSA and 0.05% sodium azide.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Restrictions
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For Research Use only
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Alternatives for antigen "SSEA-4", type "Antibodies"
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Hosts
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Rabbit (20), Mouse (5)
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Reactivities
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Human (25), Mouse (Murine) (21), Chicken (5), Dog (Canine) (5), Monkey (5), Rabbit (5), Rat (Rattus) (1)
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Applications
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Flow Cytometry (FACS) (23), Immunofluorescence (IF) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1), Western Blotting (WB) (1)
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Conjugates
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Alexa Fluor 647 (2), FITC (2), PE (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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