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SSEA-4 antibody

Antigen

SSEA-4

Clonality Monoclonal (MC-813-70)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Application
Alternatives Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Catalog no. ABIN411593
Quantity 25 ug  (0.5 mg/ml)
Price 110.00 $   Plus shipping costs $35.00
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Additional Information

Characteristics 25 ug. This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ (EG) cells) and human embryonic stem (ES) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression. Applications: Flow (Cell Surface): 10 µl/106 cells; IF, ICC, IP and Western blot.
Gene ID N/A
Immunogen Human embryonal carcinoma cell line 2102Ep
Isotype IgG3, kappa chain  (Matching secondary antibodies)
Clone MC-813-70
Description This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ (EG) cells) and human embryonic stem (ES) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

Application Details

Application Notes Flow (cell Surface), IHC, IP, Western Blot
Concentration 0.5 mg/ml
Purification Affinity Purified Antibody
Buffer 25 µg in 50 ul PBS containing 0.05% BSA and 0.05% sodium azide.
Storage Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "SSEA-4", type "Antibodies"
Hosts Rabbit (20), Mouse (5)
Reactivities Human (25), Mouse (Murine) (21), Chicken (5), Dog (Canine) (5), Monkey (5), Rabbit (5), Rat (Rattus) (1)
Applications Flow Cytometry (FACS) (23), Immunofluorescence (IF) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1), Western Blotting (WB) (1)
Conjugates Alexa Fluor 647 (2), FITC (2), PE (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)