Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) antibody

Antigen: Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)

Synonyms: APRF, HIES, FLJ20882, MGC16063, fc15d02, fl59g06, z-Stat3, MGC193054, wu:fc15d02, wu:fl59g06, STAT3, DKFZp469E1221

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411596

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-Stat3 polyclonal antibody. Reacts with human/mouse/rat. Tested in Western blot. Per researcher's feedback, the antibody also works well in IP and IHC.

Alternative name: Stat3

Gene ID: 20848

Immunogen: Synthetic peptide surrounding amino acid 73 of rat Stat3

Isotype: IgG  (Matching secondary antibodies)

Description: Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Specificity: The antibody recognizes ~97 kDa Stat3 in samples from human, mouse, and rat origins. Reactivity to other species has not been tested.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~97 kDa Stat3 in samples from human, mouse, and rat origins. Reactivity to other species has not been tested.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only