Signal Transducer and Activator of Transcription 4 (STAT4) antibody

Antigen: Signal Transducer and Activator of Transcription 4 (STAT4)

Synonyms: SLEB11, STAT4, MGC157175

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411597

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-Stat4 polyclonal antibody. Detects the 97 kDa Stat4 in samples of human/mouse/rat origins. Tested in Western blot. Other applications have not been determined. Blocking peptide (Cat.# 3471RBP-50) is available separately.

Alternative name: Stat4

Gene ID: 367264

Immunogen: Synthetic peptide surrounding amino acid 732 of rat Stat4

Isotype: IgG  (Matching secondary antibodies)

Description: Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Specificity: The antibody recognizes ~97 kDa Stat4 in samples from human, mouse, and rat origins. A ~60 kDa band can also be detected, presumably to be the cleavage fragment of Stat4.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~97 kDa Stat4 in samples from human, mouse, and rat origins. A ~60 kDa band can also be detected, presumably to be the cleavage fragment of Stat4.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Signaling, Receptors, Inflammation

Restrictions: For Research Use only