Signal Transducer and Activator of Transcription 6 (STAT6) antibody

Antigen: Signal Transducer and Activator of Transcription 6 (STAT6)

Synonyms: STAT6B, STAT6C, D12S1644, IL-4-STAT, fj66g07, wu:fj66g07, STAT6, DKFZp459D186

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411598

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-Stat6 polyclonal antibody. Detects the 119 kDa full length and the 65 kDa short form stat6 in samples from human/mouse/rat origins. Tested in Western Blot. Other applications have not been determined. Blocking peptide (Cat.# 3473BP-50) is available separately.

Alternative name: Stat6

Gene ID: 362896

Immunogen: Synthetic peptide surrounding amino acid 608 of rat Stat6

Isotype: IgG  (Matching secondary antibodies)

Description: Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Specificity: The antibody recognizes the 119 kDa full length and the 65 kDa short form of Stat6 in samples from human, mouse, and rat origins.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes the 119 kDa full length and the 65 kDa short form of Stat6 in samples from human, mouse, and rat origins.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only