Transcription Factor A, Mitochondrial (TFAM) antibody

Antigen: Transcription Factor A, Mitochondrial (TFAM)

Synonyms: TCF6, MtTF1, mtTFA, TCF6L1, TCF6L2, TCF6L3, Hmgts, tsHMG, AI661103, Mttfa, tcf6, mttf1, mttfa, tcf6l1, tcf6l2, tcf6l3, mttfa-A, xl-mtTFA, TFAM, MGC153358, zgc:153358

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411602

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-TFAM polyclonal antibody. Detects 27 kDa rat TFAM in Western blot analysis. Reacts with rat. Other applications have not been determined.

Alternative name: TFAM

Gene ID: 83474

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 225 of rat TFAM

Isotype: IgG  (Matching secondary antibodies)

Description: Transcription factor A mitochondria (TFAM) is an important activator of mitochondrial DNA transcription and replication. Studies in mice have shown that TFAM is required to regulate the mitochondrial genome copy number and is important for embryonic development. TFAM has been associated with mitochondrial disorder which includes ocular myopathy and muscular wasting in humans. Kearns-Sayre syndrome was shown in mouse model when expression of the gene was eliminated by targeted disruption in heart and muscle cells.

Application Details

Application Notes: Western blotting (0.5-4µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 27 kDa band from rat kidney tissue lysate. Reactivity to other species has not been tested.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Restrictions: For Research Use only