TLR4 antibody
| Antigen |
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
Flow Cytometry (FACS) (41), Immunoprecipitation (IP) (16), Western Blotting (WB) (16), ELISA (7), Functional Studies (Func) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (IHC) (3), Neutralization (Neut) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Blocking Antibody (Inhibition) (1), Immunofluorescence (IF) (1)
|
| Catalog no. |
ABIN411611 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
225.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Characteristics
|
100 ug affinity purified rabbit anti-TLR4 polyclonal antibody. Reacts with human, mouse, rat. Tested in Western blotting. Other applications have not been determined.
|
|
Gene ID
|
29260
|
|
Immunogen
|
Synthetic peptide corresponding to residues surrounding amino acids 819 of rat TLR4
|
|
Isotype
|
IgG (Matching secondary antibodies)
|
|
Description
|
TLR4 (Toll-like receptor 4) is a mammalain homologue of the Drosophila toll protein. The human TLR4 is a type-1 transmembrane protein containing 799 amino acid residues. The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of IL-1 receptor. TLR4 is a receptor for lipopolysaccharide (LPS). In mice that are genetically tolerant to endotoxin, mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB.
|
|
Application Notes
|
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~92 kDa TLR4 of rat origin. Reactivity to other species has not been tested.
|
|
Concentration
|
0.5 mg/ml
|
|
Purification
|
Affinity Purified Antibody
|
|
Buffer
|
100 µg (0.5 mg/ml) affinity purified rabbit anti-TLR4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
|
|
Storage
|
Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
|
|
Research Area
|
Immunology, Innate Immunity, Adaptive Immunity, Atherosclerosis, Signaling, Inflammation
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "TLR4", type "Antibodies"
|
Hosts
|
Mouse (39), Rabbit (8), Rat (5)
|
|
Reactivities
|
Human (45), Mouse (Murine) (6)
|
|
Applications
|
Flow Cytometry (FACS) (41), Immunoprecipitation (IP) (16), Western Blotting (WB) (16), ELISA (7), Functional Studies (Func) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (IHC) (3), Neutralization (Neut) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Blocking Antibody (Inhibition) (1), Immunofluorescence (IF) (1)
|
|
Conjugates
|
PE (6), Biotin (5), FITC (4), APC (2), Cy7,PE (1), RPE (1)
|
Alternatives 
