Toll-Like Receptor 5 (TLR5) antibody

Antigen: Toll-Like Receptor 5 (TLR5)

Synonyms: TIL3, SLEB1, FLJ10052, MGC126430, MGC126431, TLR5, TLR-5, tlr-5

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN411612

Additional Information

Characteristics: 100 ug affinity purified rabbit anti-TLR5 (Toll-like Receptor 5) polyclonal antibody. Reacts with human/mouse/rat. Tested in Western blot. Other applications have not been determined. Blocking peptide (Cat.# 3555RBP-50) is available separately.

Alternative name: TLR5

Gene ID: 53791

Immunogen: Synthetic peptide surrounding amino acid 829 of human TLR5

Isotype: IgG  (Matching secondary antibodies)

Description: The Toll-like receptor (TLR) family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. TLR6 is highly homologous to TLR1, and like other members of TLR family, it induces NFkB signaling upon activation. Expression of TLR receptors is highest in peripheral blood leukocytes, macrophages, and monocytes.

Specificity: The antibody recognizes ~97 kDa TLR5 in sample from human, mouse and rat origins. Reactivity to other species has not been tested. Blocking peptide (Cat.# 3555RBP-50) is available separately.

Application Details

Application Notes: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes ~97 kDa TLR5 in sample from human, mouse and rat origins. Reactivity to other species has not been tested. Blocking peptide (Cat.# 3555RBP-50) is available separately.

Concentration: 0.5 mg/ml

Purification: Affinity Purified Antibody

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-TLR5 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Storage: Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.

Research Area: Immunology, Innate Immunity, Signaling, Inflammation

Restrictions: For Research Use only

Publications

Publications: Rolli, Rosenblatt-Velin, Li et al.: "Bacterial flagellin triggers cardiac innate immune responses and acute contractile dysfunction." in: PLoS ONE, Vol. 5, Issue 9, pp. e12687, 2010 (PubMed). Further details: H9c2 cells were fixed, permeabilized with Triton X-100 0.1%, blocked with goat serum, and incubated with normal rabbit serum or a rabbit polyclonal anti-TLR5