Toll-Like Receptor 5 (TLR5) antibody

Details for Product No. ABIN411612
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Antigen
Synonyms TLR-5, sleb1, til3, tlr-5, SLEB1, TIL3
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(112), (69), (35), (1), (1)
Host
Rabbit
(101), (24), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(106), (63), (33), (33), (24), (23), (10), (8), (7), (1), (1)
Pubmed 2 references available
Quantity 100 µg
Options
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Catalog No. ABIN411612
269.50 $
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Immunogen Synthetic peptide surrounding AA 829 of human TLR5
Isotype IgG
Specificity The antibody recognizes approx. 97 kDa TLR5 in sample from human, mouse and rat origins. Reactivity to other species has not been tested. Blocking peptide is available separately.
Purification Affinity purified
Alternative Name TLR5
Background The Toll-like receptor (TLR) family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. TLR6 is highly homologous to TLR1, and like other members of TLR family, it induces NFkB signaling upon activation. Expression of TLR receptors is highest in peripheral blood leukocytes, macrophages, and monocytes.
Gene ID 53791
NCBI Accession NP_058624
Research Area Immunology, Innate Immunity, Signaling, Inflammation
Application Notes Western blotting (0.5-4 µg/mL).
However the optimal conditions should be determined individually.
Other applications have not been determined.
The antibody recognizes approx. 97 kDa TLR5 in sample from human, mouse and rat origins. Reactivity to other species has not been tested.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C/-80 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Product cited in: Rolli, Loukili, Levrand et al.: "Bacterial flagellin elicits widespread innate immune defense mechanisms, apoptotic signaling, and a sepsis-like systemic inflammatory response in mice." in: Critical care (London, England), Vol. 14, Issue 4, pp. R160, 2010 (PubMed).

Rolli, Rosenblatt-Velin, Li et al.: "Bacterial flagellin triggers cardiac innate immune responses and acute contractile dysfunction." in: PLoS ONE, Vol. 5, Issue 9, pp. e12687, 2010 (PubMed). Further details: H9c2 cells were fixed, permeabilized with Triton X-100 0.1%, blocked with goat serum, and incubated with normal rabbit serum or a rabbit polyclonal anti-TLR5

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