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Toll-Like Receptor 5 (TLR5) antibody

Details for Product No. ABIN411612, Supplier: Log in to see
Antigen
  • TLR-5
  • sleb1
  • til3
  • tlr-5
  • SLEB1
  • TIL3
Alternatives
anti-Human Toll-Like Receptor 5 antibody for Flow Cytometry
Reactivity
Human, Mouse (Murine), Rat (Rattus)
170
86
38
2
2
Host
Rabbit
151
39
4
Clonality
Polyclonal
Conjugate
Un-conjugated
16
11
9
6
5
5
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
150
84
65
30
20
15
11
6
5
5
3
1
1
1
1
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Immunogen Synthetic peptide surrounding amino acid 829 of human TLR5
Blocking Peptide Blocking peptide for this product available: ABIN412947
Isotype IgG
Specificity The antibody recognizes ~97 kDa TLR5 in sample from human, mouse and rat origins.
Reactivity to other species has not been tested.
Purification Affinity purified
Alternative Name TLR5 (TLR5 Antibody Abstract)
Background The Toll-like receptor (TLR) family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and sμggests that these receptors are involved in mediating inflammatory responses. TLR6 is highly homologous to TLR1, and like other members of TLR family, it induces NFkB signaling upon activation. Expression of TLR receptors is highest in peripheral blood leukocytes, macrophages, and monocytes.
Synonyms: TLR5 , Anti-TLR5 , FLJ10052 , MGC126430 , MGC126431 , TLR 5
Gene ID 53791
NCBI Accession NP_058624
Research Area Immunology, Innate Immunity, Signaling, Inflammation
Pathways TLR Signaling
Application Notes Western blotting (0.5-4 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-TLR5 polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Toll-Like Receptor 5 (TLR5) antibody (ABIN411612) anti-Toll-Like Receptor 5 (TLR5) antibody
Product cited in: Rolli, Rosenblatt-Velin, Li et al.: "Bacterial flagellin triggers cardiac innate immune responses and acute contractile dysfunction." in: PLoS ONE, Vol. 5, Issue 9, pp. e12687, 2010 (PubMed). Further details: H9c2 cells were fixed, permeabilized with Triton X-100 0.1%, blocked with goat serum, and incubated with normal rabbit serum or a rabbit polyclonal anti-TLR5

Rolli, Loukili, Levrand et al.: "Bacterial flagellin elicits widespread innate immune defense mechanisms, apoptotic signaling, and a sepsis-like systemic inflammatory response in mice." in: Critical care (London, England), Vol. 14, Issue 4, pp. R160, 2010 (PubMed).