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Antibodies
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anti-Toll-Like Receptor 9 (TLR9) antibody
Toll-Like Receptor 9 (TLR9) antibody
| Antigen |
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| Synonyms |
CD289 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Human, Mouse (Murine), Rat (Rattus), Pig (Porcine)
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Application
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Western Blotting (WB) (70), Flow Cytometry (FACS) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (25), Enzyme Immunoassay (EIA) (24), Immunofluorescence (IF) (11), Immunohistochemistry (IHC) (7), ELISA (5), Dot Blot (Dot) (1), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunoassay (IA) (1)
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| Catalog no. |
ABIN411614 |
| Quantity |
100 µg (0.5 mg/ml) |
| Price |
225.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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100 ug affinity purified rabbit anti-TLR9 polyclonal antibody. Reacts with human/mouse/rat/pig. Detects the 113 kDa full length and 65 kDa cleaved fragment of TLR9 in Western blot analysis. Other applications have not been determined.
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Alternative name
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TLR9
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Gene ID
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81897
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Immunogen
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Synthetic peptide surrounding amino acid 931 of mouse TLR9
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Isotype
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IgG (Matching secondary antibodies)
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Description
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The Toll-like receptor (TLR) family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR9 has been suggested as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. TLR9 is highly expressed in the spleen.
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Application Notes
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Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~113 kDa of full length and the 65 kDa cleaved fragment in Jurkat cell lysate. Ractivity to other species has not been tested.
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Concentration
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0.5 mg/ml
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Purification
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Affinity Purified Antibody
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Buffer
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100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
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Storage
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Store at -20°C. Shelf life: 12 months. The antibody solution should be gently mixed before use.
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Research Area
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Immunology, Innate Immunity, Signaling, Inflammation
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Restrictions
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For Research Use only
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Alternatives for antigen "Toll-Like Receptor 9 (TLR9)", type "Antibodies"
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Hosts
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Mouse (37), Rabbit (31), Rat (9), Chicken (2), Goat (2)
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Reactivities
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Human (63), Mouse (Murine) (41), Rat (Rattus) (15), Dog (Canine) (10), Monkey (7), Horse (Equine) (6), Primate (1)
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Applications
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Western Blotting (WB) (70), Flow Cytometry (FACS) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (25), Enzyme Immunoassay (EIA) (24), Immunofluorescence (IF) (11), Immunohistochemistry (IHC) (7), ELISA (5), Dot Blot (Dot) (1), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunoassay (IA) (1)
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Conjugates
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Biotin (8), FITC (3), PE (3), Alexa Fluor 488 (1), Alexa Fluor 647 (1)
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Epitopes
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C-Term (5), Isoform A 268-284 (5), AA 268-284 (3), cytoplasmic domain (3), AA 1050-1100 (1), Center (1), N-Term (1), internal (1)
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