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CXCL12 ELISA Kit

CXCL12 Reactivity: Human Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN414920
  • Target See all CXCL12 ELISA Kits
    CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
    Reactivity
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    3.12 pg/mL - 200 pg/mL
    Minimum Detection Limit
    3.12 pg/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SDF1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity

    This assay has high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1).
    No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.

    Cross-Reactivity (Details)
    No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.
    Sensitivity
    1.31 pg/mL
    Components
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
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    Discover our top product CXCL12 ELISA Kit
  • Application Notes
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    Comment

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Sample Volume
    100 μL
    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stromal Cell Derived Factor 1 (SDF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stromal Cell Derived Factor 1 (SDF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stromal Cell Derived Factor 1 (SDF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stromal Cell Derived Factor 1 (SDF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Reagent Preparation
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 200 pg/mL. Please prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 200 pg/mL, 100 pg/mL, 50 pg/mL, 25 pg/mL, 12.5 pg/mL, 6.25 pg/mL, 3.12 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Assay Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handling Advice
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Storage
    4 °C
    Storage Comment
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Expiry Date
    6 months
  • Chen, Li, Chen, Han, Li, Guo, Liu, Xiao: "Enhanced recruitment and hematopoietic reconstitution of bone marrow-derived mesenchymal stem cells in bone marrow failure by the SDF-1/CXCR4." in: Journal of tissue engineering and regenerative medicine, Vol. 14, Issue 9, pp. 1250-1260, (2020) (PubMed).

    Zhang, Hwang, Oh, Park, Chung, Lee, Baek, Ryoo, Woo: "Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study." in: Biomaterials, Vol. 149, pp. 77-87, (2018) (PubMed).

    Wang, Li, Qiu, Hu, Chen, Fu, Zhou, Song: "Low‑intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1‑mediated SDF‑1 expression." in: International journal of molecular medicine, Vol. 42, Issue 1, pp. 322-330, (2018) (PubMed).

    Ogłodek, Szota, Just, Szromek, Araszkiewicz: "A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity." in: Pharmacological reports : PR, Vol. 68, Issue 4, pp. 756-63, (2016) (PubMed).

    Tsai, Lin, Lin, Hsu, Wang: "High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cells and depressed shedding of vascular endothelial cells undergoing hypoxia." in: European journal of applied physiology, Vol. 116, Issue 11-12, pp. 2375-2388, (2016) (PubMed).

    Chen, Lu, Wang, Chen, Lin, Ma, Liu, Zhao, Chen: "Circulating CD133+ CD34+ progenitor cells and plasma stromal-derived factor-1alpha: predictive role in ischemic stroke patients." in: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, Vol. 24, Issue 2, pp. 319-26, (2015) (PubMed).

    Og?odek, Szota, Just, Mo?, Araszkiewicz: "The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders." in: Pharmacological reports : PR, Vol. 67, Issue 1, pp. 85-9, (2015) (PubMed).

    Meng, Huang, Di, Mu, Wang, Zhao, Zhao, Zhu, Li, Kong, Xing: "Expression of Human Epidermal Growth Factor Receptor-2 in Resected Rectal Cancer." in: Medicine, Vol. 94, Issue 47, pp. e2106, (2015) (PubMed).

    Guo, Chai, Chen, Chen, Ge, Li, Li, Li, Cao: "Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells." in: In vitro cellular & developmental biology. Animal, Vol. 51, Issue 6, pp. 578-85, (2015) (PubMed).

    Ogłodek, Szota, Moś, Araszkiewicz, Szromek: "Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder." in: Pharmacological reports : PR, Vol. 67, Issue 6, pp. 1251-8, (2015) (PubMed).

    Chiva-Blanch, Condines, Magraner, Roth, Valderas-Martínez, Arranz, Casas, Martínez-Huélamo, Vallverdú-Queralt, Quifer-Rada, Lamuela-Raventos, Estruch: "The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: a randomized clinical trial." in: Atherosclerosis, Vol. 233, Issue 2, pp. 518-24, (2014) (PubMed).

    Og?odek, Szota, Just, Mo?, Araszkiewicz: "Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression." in: Pharmacological reports : PR, Vol. 66, Issue 5, pp. 920-6, (2014) (PubMed).

    Li, Wang, Zhang, Zhao, Huang, Wu, Li, Li, Liu, Cao, Dai, Fang, Shang, Cao, Zhao, Chen: "Elevated PLGF contributes to small-cell lung cancer brain metastasis." in: Oncogene, Vol. 32, Issue 24, pp. 2952-62, (2013) (PubMed).

    Wang, Lee, Lien, Weng: "Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men." in: International journal of cardiology, Vol. 170, Issue 3, pp. 315-23, (2013) (PubMed).

    Patschan, Patschan, Henze, Blaschke, Wessels, Müller: "Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis." in: International journal of nephrology, Vol. 2012, pp. 715049, (2012) (PubMed).

    Patschan, Patschan, Temme, Korsten, Wessels, Koziolek, Henze, Müller: "Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)." in: Critical care, Vol. 15, Issue 2, pp. R94, (2011) (PubMed).

  • Target See all CXCL12 ELISA Kits
    CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
    Alternative Name
    SDF1 (CXCL12 Products)
    Synonyms
    IRH ELISA Kit, PBSF ELISA Kit, SCYB12 ELISA Kit, SDF1 ELISA Kit, TLSF ELISA Kit, TPAR1 ELISA Kit, CINC-1 ELISA Kit, Gro1 ELISA Kit, Pbsf ELISA Kit, Scyb12 ELISA Kit, Sdf1 ELISA Kit, Tlsf ELISA Kit, Tpar1 ELISA Kit, Fsp ELISA Kit, KC ELISA Kit, Mgsa ELISA Kit, N51 ELISA Kit, Scyb1 ELISA Kit, gro ELISA Kit, sdf-1 ELISA Kit, sdf1 ELISA Kit, xSDF-1 ELISA Kit, SDF-1 ELISA Kit, cxcl12 ELISA Kit, sdf-1a ELISA Kit, sdf1a ELISA Kit, wu:fa55e10 ELISA Kit, wu:fc16h12 ELISA Kit, wu:fj84c02 ELISA Kit, C-X-C motif chemokine ligand 12 ELISA Kit, C-X-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X-C motif) ligand 12 ELISA Kit, chemokine (C-X-C motif) ligand 1 ELISA Kit, C-X-C motif chemokine ligand 12 L homeolog ELISA Kit, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) ELISA Kit, CXCL12 ELISA Kit, cxcl12 ELISA Kit, Cxcl1 ELISA Kit, Cxcl12 ELISA Kit, cxcl12.L ELISA Kit, cxcl12a ELISA Kit
    UniProt
    P48061
    Pathways
    Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
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