His Tag antibody (Biotin)

Details for Product No. ABIN4276995, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
18
3
1
Host
Mouse
397
178
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
58
43
35
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
528
371
160
106
77
74
66
26
12
11
9
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
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Immunogen This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Clone 33D10-D2
Isotype IgG1
Purification Protein A purified
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:2000-1:10000, ELISA 1:50000 - 1:175000, Immunohistochemistry 1:1000-1:5000, Immunohistochemistry-Paraffin 1:1000 - 1:5000Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with 0.1 mL deionized water.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
ELISA image for anti-His Tag antibody (Biotin) (ABIN4276995) ELISA: 6-Histidine Epitope Tag Antibody (33D10.D2) [Biotin] [NBP1-42782] - ELISA of M...
Western Blotting (WB) image for anti-His Tag antibody (Biotin) (ABIN4276995) Western Blot: 6-Histidine Epitope Tag Antibody (33D10.D2) [Biotin] [NBP1-42782] - Lan...
Product cited in: Brandsma, Diao, Wang, Kohalmi, Jevnikar, Ma: "Plant-derived recombinant human serum transferrin demonstrates multiple functions." in: Plant biotechnology journal, Vol. 8, Issue 4, pp. 489-505, 2010 (PubMed).