His Tag antibody (HRP)

Details for Product No. ABIN4276996, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
24
22
14
3
2
Host
Mouse
434
184
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
58
45
36
15
14
12
11
10
10
10
10
9
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8
8
8
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4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
554
385
167
107
99
76
69
26
13
11
9
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6
6
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4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Immunogen This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Clone 33D10-D2
Isotype IgG1
Purification Protein A purified
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:1000-1:5000, ELISA 1:10000, Immunohistochemistry 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10-1:500Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with 0.1 mL deionized water to a final concentration of 1.0 mg/mL.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Buffer contains: 0.01 % Gentamicin Sulfate
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
ELISA image for anti-His Tag antibody (HRP) (ABIN4276996) ELISA: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - ELISA of Mous...
Western Blotting (WB) image for anti-His Tag antibody (HRP) (ABIN4276996) Western Blot: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - Lane 1...
ELISA image for anti-His Tag antibody (HRP) (ABIN4276996) ELISA: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - Antigen: 6X H...
Product cited in: Brandsma, Diao, Wang, Kohalmi, Jevnikar, Ma: "Plant-derived recombinant human serum transferrin demonstrates multiple functions." in: Plant biotechnology journal, Vol. 8, Issue 4, pp. 489-505, 2010 (PubMed).

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