Western Blot: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5,000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT.
ELISA: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - Antigen: 6X HIS-tagged conjugated BSA at the N or C terminus of the 6XHis. Coating amount: 0.15ug per welll. Primary antibody (direct detection): HRP conjugated 6xHIS Tag antibody diluted from stock concentration at 100ug/mL. Substrate: TMB ELISA peroxidase substrate.