Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody

Details for Product No. ABIN4330478, Supplier: Log in to see
Antigen
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Alternatives
anti-Mouse (Murine) Microtubule-Associated Protein 1 Light Chain 3 beta antibody for Electron Microscopy
Epitope
AA 1-100, N-Term
85
50
23
21
21
17
10
10
9
8
8
6
6
5
5
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
267
157
110
32
27
27
26
25
12
12
6
6
1
Host
Rabbit
250
20
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
18
15
15
14
13
13
6
5
5
5
5
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
158
121
90
76
72
67
61
37
26
10
8
6
6
3
2
1
1
1
Options
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Immunogen A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). [UniProt# Q9GZQ8].
Clone 1251A
Isotype IgG
Specificity Specific for LC3B.
Purification Protein G purified
Alternative Name LC3B/MAP1LC3B (MAP1LC3B Antibody Abstract)
Background Gene Symbol: MAP1LC3B
Gene ID 81631
UniProt Q9GZQ8
Research Area Cytoskeleton, Autophagy, Cancer
Pathways Autophagy
Application Notes Western Blot 1.0 - 2.0 μg/mL, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 10-20 μg/mL, Immunoprecipitation 2-10 μg, Immunohistochemistry-Paraffin 1:100-1:500, Flow (Intracellular) 5 μL per 1 million cellsIn Western blot this LC3B recombinant monoclonal antibody detects both LC3I and LC3II with Chloroquine treatment. With ICC autophagosome staining was observed after treatment with Chloroquine.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] -...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Immunohistochemistry-Paraffin: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - IHC anal...
Immunoprecipitation (IP) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Immunoprecipitation: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - Western blot analy...
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Western Blot: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] - Western Blot image of mon...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100), (N-Term) antibody (ABIN4330478) Immunocytochemistry/Immunofluorescence: LC3B/MAP1LC3B Antibody (1251A) [NBP2-46892] -...
Product cited in: Wechman, Rao, Cheng, Gomez-Gutierrez, McMasters, Zhou: "Development of an Oncolytic Adenovirus with Enhanced Spread Ability through Repeated UV Irradiation and Cancer Selection." in: Viruses, Vol. 8, Issue 6, 2016 (PubMed). Further details: Western Blotting

Verwey, Nolte, Joubert, Theron: "Autophagy induced by a sulphamoylated estrone analogue contributes to its cytotoxic effect on breast cancer cells." in: Cancer cell international, Vol. 16, pp. 91, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).