Tumor Necrosis Factor antibody (TNF)

Details for Product anti-TNF Antibody No. ABIN4360905, Supplier: Log in to see
Antigen
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Alternatives
anti-Human Tumor Necrosis Factor antibody for ELISA
Reactivity
Human, Mouse (Murine), Sheep (Ovine)
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460
359
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119
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Host
Mouse
934
519
184
48
10
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2
Clonality (Clone)
Monoclonal ()
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
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100
74
50
32
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13
9
4
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2
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1
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1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
887
857
621
320
286
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97
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29
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14
9
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6
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3
3
3
2
2
2
2
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2
1
1
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Options
Supplier
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Supplier Product No.
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Immunogen The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).
Clone 52B83
Isotype IgG1
Specificity This detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. Non-specific background staining is obesrved in connective tissues.
Purification Protein A or G purified
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Application Notes Western Blot 1:10-1:100, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha (17.4 kDa) is not biologically active.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS with 0.1 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Product cited in: Zhou, Robert, Kapoulea, Lei, Stagner, Jakobiec, Dohlman, Paschalis: "Sustained Subconjunctival Delivery of Infliximab Protects the Cornea and Retina Following Alkali Burn to the Eye." in: Investigative ophthalmology & visual science, Vol. 58, Issue 1, pp. 96-105, 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Rabbit).

Azeez, Olopade, Laperchia, Andrioli, Scambi, Onwuka, Bentivoglio, Olopade: "Regional Myelin and Axon Damage and Neuroinflammation in the Adult Mouse Brain After Long-Term Postnatal Vanadium Exposure." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 9, pp. 843-54, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Li, Das, Tang, Han, Jaiswal, Ray: "Transmembrane TNF-α Facilitates HIV-1 Infection of Podocytes Cultured from Children with HIV-Associated Nephropathy." in: Journal of the American Society of Nephrology : JASN, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

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