GFP antibody (Green Fluorescent Protein) (Atto 488)

Details for Product anti-GFP Antibody No. ABIN4368287, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
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Host
Camelid (Camelidae)
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Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is conjugated to Atto 488
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF)
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Options
Supplier
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Supplier Product No.
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Purpose FluoTag-Q series - high affinity monovalent binders designed for quantitative imaging
Brand FluoTag-Q
Immunogen GFP
Clone 1H1
Fragment VHH fragment
Specificity GFP, mEGFP, sfGFP and most common CFP and YFP variants
Cross-Reactivity (Details) Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
Characteristics FluoTag-Q anti-GFP, which is derived from an in-house developed single-domain antibody (sdAb) that recognizes GFP and its most common derivatives with high affinity and specificity. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95 % guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
Alternative Name GFP
Background Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse GFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of GFP fusion proteins can be exceeded by far.
Molecular Weight 27 kDa
Research Area Tags/Labels
Application Notes 1:1000 (IF, ICC)
Restrictions For Research Use only
Format Liquid
Concentration 5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light!
Storage -20 °C
Storage Comment Up to 3 months: -20 °C Up to 12 months: -80 °C or below
Supplier Images
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (Atto 488) (ABIN4368287) Stable HeLa cell line expressing YFP-fusion protein in the nuclear pore complex (A, y...
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