Recombinant GFP antibody (Atto 488)

Catalog No. ABIN4368303

Product Details anti-GFP Antibody (Atto 488)

Target: GFP (Green Fluorescent Protein (GFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Aequorea victoria

Host: Alpaca

Expression System: E.coli

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Atto 488

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Camelid sdAb anti-GFP conjugated with Atto488, Clones 1H1/1B2

Specificity: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Cross-Reactivity (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Immunogen: GFP

Clone: 1H1-1B2

Labeling Ratio: A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.

Application Details

Application Notes: Recommended dilution 1:500

Comment:

A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 5 μM

Buffer: 1.25 μM of each fluorescently labeled sdAb clone in buffered saline, 50 % glycerol, 0.09 % sodium azide. 5 μM total fluorophore concentration

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light!

Storage: -20 °C,-80 °C

Storage Comment: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Target Type: Viral Protein

Molecular Weight: 27 kDa