GFP antibody (Green Fluorescent Protein) (Abberior STAR 580)

Details for Product anti-GFP Antibody No. ABIN4368311, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
640
16
5
5
2
2
1
1
Host
Camelid (Camelidae)
343
171
80
41
15
9
8
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is conjugated to Abberior STAR 580
33
31
28
14
12
12
12
9
9
8
8
7
7
6
6
6
6
6
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF)
538
352
202
153
146
135
96
86
72
25
15
13
13
8
8
6
6
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose FluoTag-X series - Multiple fluorophores per affinity probe and multiple affinity probes per target molecule resulte in maximal brightness
Brand FluoTag-X
Immunogen GFP
Clone 1H1-1B2
Fragment VHH fragment
Specificity GFP, mEGFP, sfGFP and most common CFP and YFP variants
Cross-Reactivity (Details) Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
Characteristics FluoTag-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our FluoTag-X4 products excellent tools for all microscopy techniques.
Alternative Name GFP
Background Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse GFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of GFP fusion proteins can be exceeded by far.
Molecular Weight 27 kDa
Research Area Tags/Labels
Application Notes 1:250 (IF,ICC)
Restrictions For Research Use only
Format Liquid
Concentration 2.5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light!
Storage -20 °C
Storage Comment Up to 3 months: -20 °C Up to 12 months: -80 °C or below
Supplier Images
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (Abberior STAR 580) (ABIN4368311) 3T3 cells transfected with the GFP-tubulin (A, green), stained with FluoTag-X4 anti-G...
Did you look for something else?