Recombinant RFP antibody (Atto 488)

Catalog No. ABIN4368319

Product Details anti-RFP Antibody (Atto 488)

Target: RFP (Red Fluorescent Protein (RFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Discosoma

Host: Alpaca

Expression System: E.coli

Clonality: Monoclonal

Conjugate: This RFP antibody is conjugated to Atto 488

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Camelid sdAb anti-RFP conjugated with Atto488, Clone 2B12

Specificity: Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.

Cross-Reactivity (Details): Does not cross-react with GFP or mTagBFP derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Immunogen: RFP

Clone: 2B12

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Application Details

Application Notes: Recommended dilution 1:500

Comment:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 5 μM

Buffer: 5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light!

Storage: -20 °C,-80 °C

Storage Comment: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Expiry Date: 12 months

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP (RFP Products)

Molecular Weight: 26 kDa