RFP antibody (Red Fluorescent Protein) (Abberior STAR 580)

Details for Product anti-RFP Antibody No. ABIN4368327, Supplier: Log in to see
Antigen
Reactivity
Discosoma
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50
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Host
Camelid (Camelidae)
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Clonality (Clone)
Monoclonal ()
Conjugate
This RFP antibody is conjugated to Abberior STAR 580
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1
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF)
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Options
Supplier
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Supplier Product No.
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Purpose FluoTag-Q series - high affinity monovalent binders designed for quantitative imaging
Brand FluoTag-Q
Immunogen RFP
Clone 2B12
Fragment VHH fragment
Specificity Most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato
Cross-Reactivity (Details) Does not cross-react with GFP, mTagBFP or mTagRFP or their most common derivatives
Characteristics FluoTag-Q anti-RFP is derived from an in-house developed single-domain antibody (sdAb) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95 % guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
Alternative Name RFP (RFP Antibody Abstract)
Background Red fluorescent protein RFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse RFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of RFP fusion proteins can be exceeded by far.
Molecular Weight 26 kDa
Research Area Tags/Labels
Application Notes 1:1000 (IF, ICC)
Restrictions For Research Use only
Format Liquid
Concentration 5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light!
Storage -20 °C
Storage Comment Up to 3 months: -20 °C Up to 12 months: -80 °C or below
Supplier Images
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) antibody (Abberior STAR 580) (ABIN4368327) 3T3 cells transfected with the mCherry-tubulin and stained with FluoTag-X4 anti-RFP A...
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