RFP antibody (Red Fluorescent Protein) (Atto 542)

Details for Product anti-RFP Antibody No. ABIN4368337, Supplier: Log in to see
Antigen
Reactivity
Discosoma
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50
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Host
Camelid (Camelidae)
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39
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Clonality (Clone)
Monoclonal ()
Conjugate
This RFP antibody is conjugated to Atto 542
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3
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2
1
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF)
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Supplier Product No.
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Purpose FluoTag-X series - Multiple fluorophores per affinity probe and multiple affinity probes per target molecule resulte in maximal brightness
Brand FluoTag-X
Immunogen RFP
Clone 2B12-2A1
Fragment VHH fragment
Specificity Most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato
Cross-Reactivity (Details) Does not cross-react with GFP, mTagBFP or mTagRFP or their most common derivatives
Characteristics FluoTag-X4 anti-RFP is a blend of two in-house developed single-domain antibodies (sdAbs) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our FluoTag-X4 products excellent tools for all microscopy techniques.
Alternative Name RFP (RFP Antibody Abstract)
Background Red fluorescent protein RFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse RFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of RFP fusion proteins can be exceeded by far.
Molecular Weight 26 kDa
Research Area Tags/Labels
Application Notes 1:250 (IF,ICC)
Restrictions For Research Use only
Format Liquid
Concentration 2.5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light!
Storage -20 °C
Storage Comment Up to 3 months: -20 °C Up to 12 months: -80 °C or below
Supplier Images
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) antibody (Atto 542) (ABIN4368337) 3T3 cells transfected with the mCherry-tubulin and stained with FluoTag-X4 anti-RFP A...
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