PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (AA 200-250)

Details for Product anti-PDK4 Antibody No. ABIN446476, Supplier: Log in to see
Antigen
  • AV005916
  • pyruvate dehydrogenase kinase, isozyme 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pdk4
  • LOC100230570
  • Pdk4
  • PDK4
Alternatives
anti-Human PDK4 antibody for ELISA
Epitope
AA 200-250, Internal Region
28
20
18
17
16
12
10
10
8
6
6
6
6
5
5
4
3
2
1
1
1
1
1
1
Reactivity
Human
150
73
16
2
2
2
1
1
1
1
1
Host
Rabbit
152
20
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
11
10
8
7
7
7
4
4
4
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
152
78
60
34
24
18
4
4
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 200-250). [Swiss-Prot# Q16654]
Purification Immunogen affinity purified
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background Gene Symbol: PDK4
Molecular Weight Theoretical MW: 49 kDa
Gene ID 5166
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
Application Notes Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:500This PDK4 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~49 kDa. In ICC/IF mitochondrial staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for PDK4 Antibody Procedure Guide for NBP1-07049 - PDK4 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-PDK4 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1.24 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 200-250), (Internal Region) antibody (ABIN446476) anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 200-250), (Internal Region) antibody
Immunofluorescence (IF) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 200-250), (Internal Region) antibody (ABIN446476) Immunocytochemistry/Immunofluorescence: PDK4 Antibody [NBP1-07049] - PDK4 antibody wa...
Product cited in: Necela, Axenfeld, Serie, Kachergus, Perez, Thompson, Norton: "The antineoplastic drug, trastuzumab, dysregulates metabolism in iPSC-derived cardiomyocytes." in: Clinical and translational medicine, Vol. 6, Issue 1, pp. 5, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Did you look for something else?