Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibody

Details for Product No. ABIN447087, Supplier: Log in to see
Antigen
  • erk1/2
  • mitogen-activated protein kinase
  • erk1/2
Epitope
pThr202, pTyr204
114
103
30
30
28
19
18
17
15
15
15
15
15
12
9
9
9
8
7
7
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
403
312
300
57
24
15
9
8
8
8
7
6
5
5
3
3
2
2
2
1
1
1
1
Host
Rabbit
331
76
6
Clonality
Polyclonal
Conjugate
Un-conjugated
12
11
11
9
7
7
7
6
6
6
6
6
6
6
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
291
105
81
78
60
54
39
38
14
10
6
4
3
2
2
2
2
Options
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Immunogen Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204.
Purification Immunogen affinity purified
Alternative Name ERK1/2 (MAPK1/3 Antibody Abstract)
Background Gene Symbol: MAPK3
Molecular Weight Theoretical MW: 42 kDa
Gene ID 5595
UniProt P27361
Research Area Signaling, Inflammation
Application Notes Western Blot 1:500-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:200Western blot (WB) analysis of p-ERK1/2 (T202/Y204) pAb in extracts from Jurkat PMA cells . Immunohistochemistry (IHC) analyzes of p-ERK1/2 (T202/Y204) pAb in paraffin-embedded human breast carcinoma tissue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.2)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibody (ABIN447087) anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibody
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibody (ABIN447087) anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibody (Image 2)
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibody (ABIN447087) Western Blot: ERK1/2 [p Thr202, p Tyr204] Antibody [NBP1-19924] - Analysis of p-ERK1/...
Product cited in: Jhang, Cheng, Ho, Yen: "Monosodium urate crystals trigger Nrf2- and heme oxygenase-1-dependent inflammation in THP-1 cells." in: Cellular & molecular immunology, Vol. 12, Issue 4, pp. 424-34, 2015 (PubMed).

Huang, Cheng, Su, Lin: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, 2015 (PubMed).

Lu, Samanta, Xiang, Zhang, Hu, Chen, Bullen, Semenza: "Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 33, pp. E4600-9, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Chen, Cheng, Tzen, Cheng, Lin: "Probing relevant molecules in modulating the neurite outgrowth of hippocampal neurons on substrates of different stiffness." in: PLoS ONE, Vol. 8, Issue 12, pp. e83394, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting