Glyoxalase I (GLO1) antibody

Details for Product No. ABIN449661, Supplier: Log in to see
Antigen
  • cb554
  • zgc:66035
  • wu:fb82g09
  • glod1
  • glyi
  • glo1
  • GLO1
  • ATGLX1
  • F12F1.32
  • F12F1_32
  • GLYOXALASE I
  • glyoxalase I homolog
  • trypanothione-dependent glyoxalase I
  • 0610009E22Rik
  • 1110008E19Rik
  • 2510049H23Rik
  • AW550643
  • GLY1
  • Glo-1
  • Glo-1r
  • Glo-1s
  • Glo1-r
  • Glo1-s
  • Qglo
  • GLOD1
  • GLYI
  • glyoxalase 1
  • glyoxalase I
  • lactoylglutathione lyase
  • glyoxalase I homolog
  • glo1
  • GLO1
  • glo1-b
  • glo1-a
  • glyoxalase I
  • GLX1
  • gloA
  • Bcen_2094
  • Glo1
Reactivity
Human, Mouse (Murine)
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64
33
4
2
2
1
1
1
1
1
1
1
Host
Mouse
68
38
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
81
53
36
32
20
13
11
9
8
4
3
2
1
1
1
Options
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Immunogen Full length human GLO1 protein [Swiss-Prot #Q04760].
Clone Glo1a
Isotype IgG1 kappa
Purification Protein G purified
Alternative Name Glyoxalase I (GLO1 Antibody Abstract)
Background Gene Symbol: GLO1
Molecular Weight Theoretical MW: 21 kDa
Gene ID 2739
UniProt Q04760
Application Notes Western Blot 1.0 - 2.0 μg/mL, Simple Western 1:50, ELISA 0.15 ng/ 1 μg protein, Immunocytochemistry/Immunofluorescence 10 μg/mL, ImmunoprecipitationThis GLO1 antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 21 kDa. Immunoprecipitation was reported in scientific literature. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for GLO1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-GLO1 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.Immunocytochemistry Protocol Immunocytochemistry Protocol
. Cells are cultured one day prior to the experiment.
. After washing twice with PBS and they are fixed with 4 % paraformaldehyde in PBS at ?20C for 15 min.
. Followed by two washes with PBS, they are permeabilized with 0.1 %Triton X-100 in PBS at ?20C for 5 min.
. To remove the detergent the cells are washed5 times with PBS and then blocked with 2.5 % goat serum in PBS for 2 hr at RT.
. Cells are then incubated with GLOI mAb (10 μ,g/ml) in PBS for 1 hr at RT and washed twice for 5 min with PBS.
. Cells are incubated with secondary antibody (anti-mouseIgG) conjugated with Texas Red (1: 400 dilution in PBS) (Molecular Probes) for 1 hr at RT.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-Glyoxalase I (GLO1) antibody (ABIN449661) anti-Glyoxalase I (GLO1) antibody
Western Blotting (WB) image for anti-Glyoxalase I (GLO1) antibody (ABIN449661) anti-Glyoxalase I (GLO1) antibody (Image 2)
Simple Western (SimWes) image for anti-Glyoxalase I (GLO1) antibody (ABIN449661) Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view...
Simple Western (SimWes) image for anti-Glyoxalase I (GLO1) antibody (ABIN449661) Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view...
Product cited in: Zeng, Zhang, Huang, Vedantham, Rosario, Ananthakrishnan, Yan, Ramasamy, DeMatteo, Emond, Friedman, Schmidt: "Opposing roles of RAGE and Myd88 signaling in extensive liver resection." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 2, pp. 882-93, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Mailankot, Padmanabha, Pasupuleti, Major, Howell, Nagaraj: "Glyoxalase I activity and immunoreactivity in the aging human lens." in: Biogerontology, Vol. 10, Issue 6, pp. 711-20, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation