His Tag antibody

Details for Product No. ABIN449792, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
18
3
1
Host
Goat
398
178
26
23
4
4
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
58
44
35
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
528
371
159
105
77
75
65
26
12
11
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H
Isotype IgG
Specificity This antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. This antibody may not detect all N-terminal (NT) histidine tagged proteins.
Purification Immunogen affinity purified
Alternative Name 6xHistidine Epitope Tag (HHHHHH)
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:1000-1:10000, ELISA 1:1000 - 1:30000, Immunocytochemistry/Immunofluorescence 1:100-1:400, Immunoprecipitation 1 - 4 μg/mg lysate
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN449792) Western Blot: 6-Histidine Epitope Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E...
Product cited in: Cabrita, Jäggi, Widjaja, Christofori: "A functional interaction between sprouty proteins and caveolin-1." in: The Journal of biological chemistry, Vol. 281, Issue 39, pp. 29201-12, 2006 (PubMed).