His Tag antibody

Details for Product No. ABIN449792, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
24
22
14
3
2
Host
Goat
435
184
26
23
4
4
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
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7
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4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
554
385
166
106
99
77
68
26
13
11
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H
Isotype IgG
Specificity This antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. This antibody may not detect all N-terminal (NT) histidine tagged proteins.
Purification Immunogen affinity purified
Alternative Name 6xHistidine Epitope Tag (HHHHHH)
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:1000-1:10000, ELISA 1:1000 - 1:30000, Immunocytochemistry/Immunofluorescence 1:100-1:400, Immunoprecipitation 1 - 4 μg/mg lysate
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN449792) Western Blot: 6-Histidine Epitope Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E...
Product cited in: Cabrita, Jäggi, Widjaja, Christofori: "A functional interaction between sprouty proteins and caveolin-1." in: The Journal of biological chemistry, Vol. 281, Issue 39, pp. 29201-12, 2006 (PubMed).

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