KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1) antibody

Antigen: KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1)

Clonality: Monoclonal (KR-10)

Host: Mouse

Reactivity: Various Species

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN452266

Additional Information

Characteristics: Synonyms: KDEL endoplasmic reticulum protein retention receptor 1, ERD2.1, ER marker, ER lumenprotein retaining receptor 1, Putative MAPK-activating protein PM23

Alternative name: KDEL Receptor 1 / KDELR1

Gene ID: 618184

Swiss-Prot: P33946

Immunogen: A 21 residue synthetic peptide based on the bovine KDEL receptor and the peptide coupledto KLH

Cross-Reactivity: Various Species

Isotype: IgG1  (Matching secondary antibodies)

Clone: KR-10

Description: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, thetransportation system of the eukaryotic cell. The majority of endoplasmic reticulumresident proteins are retained in the endoplasmic reticulum through a retention motif. Thismotif is composed of four amino acids at the C-terminal end of the protein sequence. Themost common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDELdoes occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree ofsequence identity, and all are located within the cis-Golgi and its intermediatecompartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1,which is involved in COPI dependent vesicle transport, and the KDEL receptor may also beresponsible for the recruitment of this ARF1 to membranes which can then aid in theregulation of vesicle budding (3). It is also important to note that the KDEL receptorexhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for theyeast ER retention signal (4, 5).

Specificity: This antibody detects ~25kDa protein corresponding to the molecular mass of KDEL onSDS-PAGE immunoblots. Species Reactivity: Tested: Human, Monkey, Mouse, Rat, Hamster, Rabbit, Pig, Bovine, Sheep, Canine,Chicken, Drosphilia, Xenopus

Application Details

Application Notes: Immunoprecipitation (1). Immunocytochemistry (3). Immunohistochemisztry on frozen sections. Western blot (2).

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS pH 7.2, in 50% glycerol with 0.09% azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only