CACNA1H antibody (AA 1019-1293)

Catalog No. ABIN452269

Product Details anti-CACNA1H Antibody

Target: CACNA1H (Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H))

Binding Specificity: AA 1019-1293

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CACNA1H antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Specificity: This antibody detects Cav3.2 Ca2+ Channel at ~260 kDa. No cross-reactivity against Cav1.3.

Cross-Reactivity (Details): Species reactivity (tested):Human

Characteristics: Synonyms: Cav3.2, Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gatedcalcium channel subunit alpha Cav3.2, Low-voltage-activated calcium channel alpha1 3.2subunit

Purification: Protein G chromatography

Immunogen: Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3. 2 (also known asvoltagedependent T-type calcium channel subunit alpha-1H

Clone: S55-10

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 - 10 μg/mL (if results are off, try using the lysate without boiling). Immunoprecipitation. Immunocytochemistry: 0.1 - 1.0 μg/mL (Perox).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CACNA1H

Target: CACNA1H (Calcium Channel, Voltage-Dependent, T Type, alpha 1H Subunit (CACNA1H))

Alternative Name: CACNA1H (CACNA1H Products)

Synonyms: CACNA1HB antibody, Cav3.2 antibody, ECA6 antibody, EIG6 antibody, MNCb-1209 antibody, alpha13.2 antibody, calcium voltage-gated channel subunit alpha1 H antibody, calcium channel, voltage-dependent, T type, alpha 1H subunit antibody, CACNA1H antibody, Cacna1h antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, Cav3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (3, 4). Studies also suggest that the up-regulations of Cav3.2 may participate in the progression of prostate cancer toward an androgen-independent stage (5).Synonyms: Cav3.2, Low-voltage-activated calcium channel alpha1 3.2 subunit, Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2

Gene ID: 8912

UniProt: O95180

Pathways: C21-Steroid Hormone Metabolic Process