PAR1 antibody (Internal Region)

Catalog No. ABIN452298

Product Details anti-PAR1 Antibody

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes F2R/PAR1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TRARRPESKATNAT, from the Internal region (near N-terminus) of the protein sequence

Application Details

Application Notes: Peptide ELISA: Detection limit dilution: 1/32000. Western blot: 0.5-2 μg/mL. Approx 48 kDa band observed in lysates of cell line HeLa(calculated MW of 47.8 kDa according to NP_001983.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: Thrombin Receptor / F2R (F2R Products)

Synonyms: CF2R antibody, HTR antibody, PAR-1 antibody, PAR1 antibody, TR antibody, Par1 antibody, TRGPC antibody, AI482343 antibody, Cf2r antibody, ThrR antibody, Par-1 antibody, f2r-a antibody, par1 antibody, coagulation factor II thrombin receptor antibody, coagulation factor II (thrombin) receptor antibody, coagulation factor 2 (thrombin) receptor L homeolog antibody, F2R antibody, F2r antibody, f2r.L antibody

Background: Thrombin receptor is a 7 transmembrane G protein coupled receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. It may play a role in platelet activation and in vascular development. Thrombin receptor expression has been documented in various blood cells, colon, kidney, liver, lung, skin, and vessel. ESTs have been isolated from adrenal, embryo, heart, heart/melanocyte/uterus, liver/spleen, pineal, skin, spleen, and vessel.Synonyms: CF2R, Coagulation factor II receptor, PAR-1, PAR1, Proteinase-activated receptor 1

Gene ID: 2149

NCBI Accession: NP_001983

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus