SCN9A antibody (C-Term)

Catalog No. ABIN452415

Product Details anti-SCN9A Antibody

Target: SCN9A (Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A))

Binding Specificity: AA 1751-1946, C-Term

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCN9A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: This antibody detects SCN9A / PN1 at ~230 kDa. No cross reactivity against other Nav channels observed.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Characteristics: Synonyms: NENA, NE-Na, Sodium channel protein type 9 subunit alpha, Sodium channel protein typeIX subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.7, Neuroendocrinesodium channel, Peripheral sodium channel 1

Purification: Protein G Chromatography

Immunogen: Fusion protein amino acids 1751-1946 (C-terminus) of Human SCN9A / PN1

Clone: S68-6

Isotype: IgG1

Application Details

Application Notes: Western blot: 1-10 μg/mL (if results are off, try using the lysate without boiling). Immunprecipitaion. Immunocytochemistry: 0.1-1.0 μg/mL (Perox).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SCN9A

Target: SCN9A (Sodium Channel, Voltage-Gated, Type IX, alpha Subunit (SCN9A))

Alternative Name: SCN9A / PN1 (SCN9A Products)

Synonyms: SCN9A antibody, DKFZp459G084 antibody, Nav1.7 antibody, PN1 antibody, mKIAA4197 antibody, Scn2a antibody, ETHA antibody, FEB3B antibody, GEFSP7 antibody, NE-NA antibody, NENA antibody, SFNP antibody, sodium channel, voltage-gated, type IX, alpha subunit antibody, sodium voltage-gated channel alpha subunit 9 antibody, sodium channel protein type 9 subunit alpha antibody, sodium channel, voltage-gated, type IX, alpha antibody, SCN9A antibody, LOC100016648 antibody, Scn9a antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Nav1.7 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.7 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.7 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).Synonyms: NE-Na, NENA, Neuroendocrine sodium channel, Peripheral sodium channel 1, Sodium channel protein type 9 subunit alpha, Sodium channel protein type IX subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.7

Gene ID: 6335

UniProt: Q15858