Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CAMK2A antibody (Internal Region)

CAMK2A Reactivity: Human, Mouse, Rat, Dog WB, IHC (p), EIA Host: Goat Polyclonal unconjugated
Catalog No. ABIN452427
  • Target See all CAMK2A Antibodies
    CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
    Binding Specificity
    • 16
    • 12
    • 11
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 76
    • 55
    • 54
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog
    Host
    • 85
    • 28
    Goat
    Clonality
    • 78
    • 35
    Polyclonal
    Conjugate
    • 60
    • 8
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    This CAMK2A antibody is un-conjugated
    Application
    • 86
    • 57
    • 31
    • 24
    • 21
    • 19
    • 15
    • 12
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Sequence
    c-PRTAQSEETR VWHR
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).
    Cross-Reactivity (Details)
    Species reactivity (expected):Rat, Canine.
    Species reactivity (tested):Human, Mouse.
    Purification
    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
    Immunogen
    Peptide with sequence from the internal region of the protein sequence
    Top Product
    Discover our top product CAMK2A Primary Antibody
  • Application Notes
    Peptide ELISA: 1/64000. Western blot: 0.1 - 0.3 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
    Alternative Name
    CAMK2A (CAMK2A Products)
    Synonyms
    camk2 antibody, camk2b antibody, zgc:112538 antibody, zgc:123320 antibody, CAMKA antibody, CaMKII antibody, R74975 antibody, mKIAA0968 antibody, PK2CDD antibody, PKCCD antibody, calcium/calmodulin dependent protein kinase II alpha antibody, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antibody, calcium/calmodulin-dependent protein kinase II alpha antibody, CAMK2A antibody, camk2a.S antibody, camk2a antibody, Camk2a antibody
    Background
    Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1 % of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30 % of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968
    Gene ID
    815
    NCBI Accession
    NP_057065
    UniProt
    Q9UQM7
    Pathways
    WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
You are here:
Support