F7
Reactivity: Human
WB, ELISA
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA. Clone RFFVII/2 may be used as a Capture antibody in Immunoassay for Factor VII incombination with clone RFFVII/1 as a Detection reagent. Radioimmunoassays. Western blotting: This Factor VII antibody binds to a conformational epitope and will notbind to reduced Factor VI. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Factor VII is a 50 kDa multi-domain, single chain plasma glycoprotein synthesised in the liver and having anormal circulating concentration of between 400 and 600 μg/L. It is essential for blood coagulation. Factor VII is converted to the active, two chain, serine protease Factor VIIa through cleavage by thrombin, factor Xa, factor IXa or factor XIIa. Factor VIIa converts factor X to factor Xa, which in turn converts Prothrombin to Thrombin.Synonyms: Proconvertin, SPCA, Serum prothrombin conversion accelerator