FAS antibody (Internal Region)

Catalog No. ABIN452677

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FAS antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Anti-FAS/CD95 antibody is expected to recognize reported isoforms 1, 2 and 3.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KTCRKHRKENQGSH from the internal region of the protein sequence according to NP_000034.1, NP_690610.1, NP_690611.1.

Application Details

Application Notes: Peptide ELISA: Detection limit dilution 1/32000. Western blot: 0.1-0.3 μg/mL. Approx 37 kDa band observed in lysates of cell line MOLT4(calculated MW of 37.7 kDa according to NP_000034.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Synonyms: ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody

Background: CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6

Gene ID: 355

NCBI Accession: NP_000034

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity