Ghrelin antibody (C-Term)

Catalog No. ABIN452691

Product Details anti-Ghrelin Antibody

Target: Ghrelin (GHRL)

Binding Specificity: C-Term

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Ghrelin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Specificity: This antibody is expected to recognise all reported isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDILWEEAKEAPADK, from the C Terminus of the protein sequence according to NP_057446.1, NP_001128413.1, NP_001128416.1, NP_001128417.1, NP_001128418.1.

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Immunohistochemistry: 2-5 μg/mL. In paraffin embedded Human Placenta shows stainingof trophoblast cytoplasm.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Ghrelin

Target: Ghrelin (GHRL)

Alternative Name: Ghrelin / GHRL (GHRL Products)

Synonyms: 2210006E23Rik antibody, Ghr antibody, MTLRP antibody, MTLRPAP antibody, m46 antibody, Gh1-1 antibody, GRHL antibody, MTLRP-AP antibody, GHR antibody, ghrelin antibody, ghrelin and obestatin prepropeptide antibody, ghrelin/obestatin prepropeptide antibody, Ghrl antibody, GHRL antibody, ghrl antibody

Background: Ghrelin was originally discovered as a preprohormone that stimulates the release of growth hormone from the anterior pituitary. Recent studies have shown that ghrelin, in concert with other hormones, helps to control both appetite and energy balance. The synthesis of Ghrelin takes place predominantly in epithelial cells of the stomach, although it is also produced in smaller quantities in the placenta, kidney, pituitary and hypothalamus. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release from the pituitary. It has an appetite stimulating effect, induces adiposity, and stimulates gastric acid secretion, and it is involved in growth regulation. Ghrelin is synthesized as a preprohormone (13 kDa), then proteolytically processed to yield a 28 amino acid peptide (3 kDa). Obestatin, an appetite suppressing peptide hormone, is also produced in the process. An interesting and unique modification is imposed on the ghrelin hormone during synthesis in the form of octanoic acid bound to one of its amino acids. This modification is necessary for biological activity.Synonyms: Appetite-regulating hormone, Ghrelin-27, Ghrelin-28, Growth hormone secretagogue, Growth hormone-releasing peptide, M46 protein, MTLRP, Motilin-related peptide, Obestatin

Gene ID: 51738

NCBI Accession: NP_001128413

UniProt: Q9UBU3

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour