3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) antibody

Antigen: 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN453103

Additional Information

Characteristics: Synonyms: 3-hydroxy-3-methylglutaryl-coenzyme A reductase

Alternative name: HMG-CoA reductase / HMGCR

Gene ID: 3156

Swiss-Prot: P04035

Immunogen: KLH conjugated synthetic peptide selected from the Center region of human HMGCR

Cross-Reactivity: Human

Description: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulatedvia a negative feedback mechanism mediated by sterols and non-sterol metabolitesderived from mevalonate, the product of the reaction catalyzed by reductase. Normally inmammalian cells this enzyme is suppressed by cholesterol derived from the internalizationand degradation of low density lipoprotein (LDL) via the LDL receptor. Competitiveinhibitors of the reductase induce the expression of LDL receptors in the liver, which in turnincreases the catabolism of plasma LDL and lowers the plasma concentration ofcholesterol, an important determinant of atherosclerosis.

Specificity: This antibody detects HMGCR at Center. Species Reactivity: Tested: HumanAdd. Information: Molecular weight: 97476 Da

Application Details

Application Notes: Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Buffer: PBS with 0.09% (W/V) sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only