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Antibodies
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anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr492) antibody
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr492) antibody
| Antigen |
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| Synonyms |
SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (79), Flow Cytometry (FACS) (37), Immunohistochemistry (IHC) (33), Immunofluorescence (IF) (32), ELISA (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Enzyme Immunoassay (EIA) (11), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN453275 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
285.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: ZAP-70, ZAP 70, SRK, 70 kDa zeta-associated protein, Syk-related tyrosine kinase
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Alternative name
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ZAP70
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Gene ID
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7535
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Swiss-Prot
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P43403
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Immunogen
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KLH conjugated synthetic phosphopeptide corresponding to amino acid residuessurrounding Y492 of human ZAP70
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Cross-Reactivity
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Human
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Description
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ZAP70 is an enzyme belonging to the protein tyrosine kinase family, and it plays a role inT-cell development and lymphocyte activation. This enzyme, which is phosphorylated ontyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initialstep of TCR-mediated signal transduction in combination with the Src family kinases, Lckand Fyn. This enzyme is also essential for thymocyte development.
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Specificity
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This antibody reacts to Y492 phosphorylated ZAP70. Species: Human. Others not tested.
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Application Notes
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ELISA: 1/1,000. Dot blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.25 mg/ml
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Buffer
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PBS with 0.09% (W/V) sodium azide
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", type "Antibodies"
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Hosts
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Rabbit (99), Mouse (42)
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Reactivities
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Human (136), Mouse (Murine) (66), Rat (Rattus) (60), Cow (Bovine) (18), Pig (Porcine) (18), Dog (Canine) (17), Horse (Equine) (16), Rabbit (16), Chicken (15), Guinea Pig (15)
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Applications
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Western Blotting (WB) (79), Flow Cytometry (FACS) (37), Immunohistochemistry (IHC) (33), Immunofluorescence (IF) (32), ELISA (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Enzyme Immunoassay (EIA) (11), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (6), FITC (6), PE (3), HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alkaline Phosphatase (AP) (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
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Epitopes
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pTyr319 (10), pTyr493 (8), Ab-319 (5), Ab-493 (5), C-Term (5), Center (3), Ab-292 (1), Ab-315 (1), pTyr292 (1), pTyr315 (1)
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Alternatives 
