V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (Avian) (RELA) (N-Term) antibody

Antigen: V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (Avian) (RELA)

Synonyms: p50, KBF1, p105, EBP-1, MGC54151, NFKB-p50, NF-kappaB, NFKB-p105, NF-kappa-B, DKFZp686C01211, NF-kB, NF-KB1, NFKB1, kbf1, ebp-1, MGC86262, nfkb-p50, nf-kappaB, nfkb-p105, nf-kappa-b

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN453343

Additional Information

Characteristics: Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3, Nuclear factorNF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer inB-cells 3

Alternative name: RELA / NF-kB p65

Gene ID: 5970

Swiss-Prot: Q04206

Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of human RELA

Cross-Reactivity: Human

Description: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL(MIM 164910), RELA, or RELB(MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is themost abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins(NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in thecytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases(IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via theubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKBcomplex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R isan A or G purine, and Y is a C or T pyrimidine).

Specificity: This antibody reacts to NF-kB p65 / RELA. Species: Human. Others not tested.

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Buffer: PBS with 0.90% (W/V) sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only