ADAM Metallopeptidase Domain 30 (ADAM30) (C-Term) antibody

Antigen: ADAM Metallopeptidase Domain 30 (ADAM30)

Synonyms: svph4, MGC132801, MGC132802, 4933424D07Rik

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN453499

Additional Information

Characteristics: Synonyms: Disintegrin and metalloproteinase domain-containing protein 30

Alternative name: ADAM30

Gene ID: 11085

Swiss-Prot: Q9UKF2

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the C-terminal region of human ADAM30.

Cross-Reactivity: Human

Description: ADAM30 was first cloned along with ADAM29 from human testis cDNA, in a search for novelADAMs. The ADAM29 and ADAM30 sequences are dissimilar, and share 37% identity onthe amino acid level. Two different ADAM30 sequences were identified in the initial paper,790 amino acids and 781 amino acids. The 9 amino acid deletion in the shorter form is inthe cytoplasmic domain, and may affect interaction with cytoplasmic elements. A memberof the metalloproteinase family containing disintegrin-like domains (ADAMs), the functionof ADAM23 is still poorly understood. ADAM30 contains the canonical HExxHxxxxxH zincmetalloproteinase motif, and is not thought to be proteolytically active. ADAM29 does nothave this conserved zinc binding motif, and it is tempting to speculate that the twoproteins form a heterodimer, such as ADAM1 and ADAM2 do (although there is no directevidence to date for such an interaction). Other ADAMs family members (ADAM10,ADAM17) have been more thoroughly studied, and are known to play key roles ininflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM30 sequence codes for a 790 amino acid protein, containing a Type Itransmembrane domain, with a predicted mass of 88. 94 kDa and a pI of 8. 16. The shorterform is 781 amino acids, with predicted mass of 88 kDa and 8. 50 pI.

Specificity: This antibody is specific to ADAM30 (C-term). Species Reactivity: Tested: Human.

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100~1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Buffer: PBS containing 0.09% (W/V) Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Restrictions: For Research Use only