Adeno-Associated Virus 2 (AAV-2) (intact particle) antibody (Biotin)

Antigen: Adeno-Associated Virus 2 (AAV-2) (intact particle)

Clonality: Monoclonal (A20)

Host: Mouse

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Catalog no.: ABIN453888

Additional Information

Characteristics: Synonyms: AAV2

Immunogen: Adeno-associated virus capsid proteins and virus particles

Isotype: IgG3

Clone: A20

Specificity: A20 specifically reacts with intact adeno-associated virus particles, empty and fullcapsids. Recognizes a conformational epitope of assembled capsids, not present indenatured capsid proteins and native but unassembled capsid proteins. Species: Human, Monkey. Others not tested.

Application Details

Application Notes: Immunofluorescence: Overnight at 2-8°C. Immunohistochemistry: Overnight at 2-8°C. ELISA: 1/20, use PBS with 0.05% Tween 20, 1 h at 37°C. The antibody cannot be used for immunoblotting. Epitope mapping experiments (Wobus et al. , see below) identified four immunoreactive(discontinous) regions. The major reaction was attributed to sequence aa 369 to aa378 ofAAV-2 capsids. The antibody is also useful for neutralizing experiments. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 20 µg/ml

Storage: After reconstitution store the antibody at 2 - 8°C. Shelf life: 6 weeks from despatch.

Restrictions: For Research Use only