WNT9B antibody (Internal Region)

Catalog No. ABIN453971

Product Details anti-WNT9B Antibody

Target: WNT9B (Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This WNT9B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Recognizes WNT15/WNT9B.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRGNKDLRARADA, from the internal region of Human WNT9B (WNT15) according to NP_003387.1.

Application Details

Application Notes: Peptide ELISA: WNT9B Antibody Detection limit dilution 1/8000. Western blot: 0.3-1 μg/mL. Approx 38 kDa band observed in Human Brain Cerebellumlysates (calculated MW of 38 kDa according to NP_003387.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for WNT9B

Target: WNT9B (Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B))

Alternative Name: WNT9B (WNT9B Products)

Synonyms: WNT14B antibody, WNT15 antibody, WNT9B antibody, wnt-9b antibody, Wnt14b antibody, Wnt15 antibody, clf antibody, clf1 antibody, wnt-14b antibody, wnt-15 antibody, Wnt family member 9B antibody, wingless-type MMTV integration site family member 9B L homeolog antibody, protein Wnt-9b antibody, wingless-type MMTV integration site family, member 9B antibody, WNT9B antibody, wnt9b.2.L antibody, Wnt9b antibody, LOC468296 antibody, wnt9b antibody

Background: Wnt9b is a member of the WNT family and is a ligand for members of the frizzled family of seven transmembrane receptors. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. The gene is clustered with WNT3, another family member, in the chromosome 17q21 region.Synonyms: Protein Wnt-9b, UNQ6973/PRO21956, WNT14B, WNT15, Wnt-14b, Wnt-15

Gene ID: 7484

NCBI Accession: NP_003387

UniProt: O14905

Pathways: WNT Signaling, Tube Formation