IREB2 antibody (Internal Region)

Catalog No. ABIN453972

Product Details anti-IREB2 Antibody

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IREB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: c-SIHYEGSEYK LSHGS

Specificity: This antibody recognizes Iron Regulatory Protein 2 (IREB2/IRP2).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of IREB2 (IRP2) according to NP_004127.1. Genename: IREB2

Application Details

Application Notes: Peptide ELISA: IREB2 Antibody Detection limit dilution 1/32000. Western blot: 1-3 μg/mL. Approx 110 kDa band observed in Human Liver lysates (calculatedMW of 100 kDa according to NP_004127.1). An additional band of unknown identity wasalso consistently observed at 60 kDa. This band was successfully blocked by incubationwith the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IREB2

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Alternative Name: IREB2 (IREB2 Products)

Synonyms: IREB2 antibody, im:7153062 antibody, DKFZp468N047 antibody, D9Ertd85e antibody, Irp2 antibody, ACO3 antibody, IRP2 antibody, IRP2AD antibody, IREBP2 antibody, iron responsive element binding protein 2 antibody, iron-responsive element binding protein 2 antibody, iron responsive element binding protein 2 L homeolog antibody, IREB2 antibody, ireb2 antibody, Ireb2 antibody, ireb2.L antibody

Background: Iron regulatory protein 2 (IREB2 or IRP2) is a central regulator of cellular iron homeostasis due to its regulation of specific mRNAs encoding proteins of iron uptake and storage. In mammalian cells, regulation of the expression of proteins involved in iron metabolism is achieved through interactions of iron-sensing proteins known as iron regulatory proteins (IRPs), with transcripts that contain RNA stem-loop structures referred to as iron responsive elements (IREs). Two distinct but highly homologous proteins, IRP1 and IRP2, bind IREs with high affinity when cells are depleted of iron, inhibiting translation of some transcripts, such as ferritin, or turnover of others, such as the transferrin receptor (TFRC). IRPs sense cytosolic iron levels and modify expression of proteins involved in iron uptake, export and sequestration according to the needs of individual cells. Regulating the amount of iron sequestered by ferritin and taken up by transferrin receptor ensures that cells acquire sufficient iron for their needs, while preventing iron toxicity.Synonyms: IRE-BP2, IRP2, Iron regulatory protein 2, Iron-responsive element-binding protein 2

Gene ID: 3658

NCBI Accession: NP_004127

UniProt: P48200

Pathways: Transition Metal Ion Homeostasis